You are here: Home > Senior Research Technician – Deep Mutational Scanning of Membrane ProteinsSenior Research Technician – Deep Mutational Scanning of Membrane ProteinsThe InstituteThe Centre for Genomic Regulation (CRG) is an international biomedical research institute of excellence, based in Barcelona, Spain, with more than 400 scientists from 44 countries. The CRG is composed by an interdisciplinary, motivated and creative scientific team which is supported both by a flexible and efficient administration and by high-end and innovative technologies.In April 2021, the Centre for Genomic Regulation (CRG) received the renewal of the ' HR Excellence in Research ' Award from the European Commission. This is a recognition of the Institute's commitment to developing an HR Strategy for Researchers, designed to bring the practices and procedures in line with the principles of the European Charter for Researchers and the Code of Conduct for the Recruitment of Researchers ( Charter and Code ).We are looking to hire a Senior Research Technician join a large-scale project performing deep mutational scanning of membrane proteins. The role involves variant library design, library generation, selections in human cell lines, DNA sequencing library generation and data analysis.About the labThe focus of Lehner lab is using massively parallel selection experiments to tackle the fundamental encoding problems of molecular biology. Towards this goal we have developed, benchmarked, and applied at scale experimental methods that use DNA synthesis-selection-sequencing to perform biophysical measurements including quantifying how changes in sequence alter protein stability, binding affinity, aggregation, allostery and expression. We have used these methods to produce the first comprehensive maps of long-range allsoteric communication in proteins, to quantify the effects of > 500,000 variants in >500 human proteins, and to probe the genetic architecture of proteins, protein interactions, and RNAs.



A key component of this work has been the development of computational methods for analyzing deep mutational scanning data and for fast and adaptable fitting of mechanistic and statistical models to the data. Our current focus is on generating datasets of sufficient size and diversity to train machine learning models to accurately predict how changes in sequence alter molecular biology.Whom would we like to hire?Professional experienceMust HaveYou have extensive experience in molecular biology including large-scale data generation and, ideally, deep mutational scanningYou have extensive experience in the analysis of sequencing datasetsDesirable but not required/ Nice to haveExperience in statistical data analysis and bioinformaticsExperience in high-throughput genomicsEducation and trainingYou hold a PhD or master’s degree in molecular biology or a related disciplineLanguagesYou are proficient in EnglishTechnical skillsYou have advanced molecular biology skillsYou have developed and optimised new experimental protocolsYou keep meticulous recordsCompetencesYou can work in an interdisciplinary teamYou can plan, optimise and execute complex protocolsYou can work independently and have experience of project managementYou have very good organisational skillsThe Offer – Working ConditionsContract duration: Temporary contract, 6 monthsEstimated annual gross salary: Salary is commensurate with qualifications and consistent with our pay scalesTarget start date: October 2026We provide a highly stimulating environment with state-of-the-art infrastructures, and unique professional career development opportunities. To check out our training and development portfolio, please visit our website in the training section .We offer and promote a diverse and inclusive environment and welcomes applicants regardless of age, disability, gender, nationality, ethnicity, religion, sexual orientation or gender identity.The CRG is committed to reconcile a work and family life of its employees and are offering extended vacation period and the possibility to benefit from flexible working hours.#J-18808-Ljbffr

📌 Senior Research Technician – Deep Mutational Scanning Of Membrane Proteins (Madrid)
🏢 Centre For Genomic Regulation
📍 Madrid