Organisation/Company FUNDACION INSTITUTO INVESTIGACION SANITARIA FUNDACION JIMENEZ DIAZ Research Field Biological sciences Other Researcher Profile First Stage Researcher (R1) Positions Master Positions Application Deadline 2 Sep 2026 - 15:15 (Europe/Madrid) Country Spain Type of Contract Temporary Job Status Full-time Hours Per Week 40 Offer Starting Date 27 Aug 2026 Is the job funded through the EU Research Framework Programme? Not funded by a EU programme Is the Job related to staff position within a Research Infrastructure? No
Offer Description
Researcher for carrying out the prime editing experiments necessary for the publication of the article entitled “Highly Efficient Prime Editing Correction of a Prevalent Frameshift COL7A1 Mutation in Recessive Dystrophic Epidermolysis Bullosa
Graduate in Biology and Master's degree in Biotechnology
Specific Requirements
Experience in developing and optimizing Base and Prime editing as gene editing strategies.




Experience in the culture and maintenance of primary cells and cell lines
Experience in the purification and isolation of skin cells from human and murine primary samples (skin biopsies).
Experience in the molecular biology techniques, including plasmid amplification, cloning, genomic DNA, plasmid, protein, and RNA extraction, PCR, qPCR, ddPCR, Western blot, and in vitro messenger RNA synthesis.
Experience in the characterization of generated mutations using next-generation sequencing and Sanger sequencing.
Experience in the development of bioengineered skin equivalents.
Experience in histological tissue processing, sectioning, and immunohistochemistry and immunofluorescence techniques.
Experience in writing and orally presenting experimental results in English.
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📌 Predoctoral Researcher, Advanced Therapies (España)
🏢 Fundación Instituto Investigación Sanitaria Fundación Jimenez Diaz
📍 España