Organisation/Company FUNDACION INSTITUTO INVESTIGACION SANITARIA FUNDACION JIMENEZ DIAZ Research Field Biological sciences Other Researcher Profile First Stage Researcher (R1) Positions Master Positions Application Deadline 2 Sep 2026 - 15:15 (Europe/Madrid) Country Spain Type of Contract Temporary Job Status Full-time Hours Per Week 40 Offer Starting Date 27 Aug 2026 Is the job funded through the EU Research Framework Programme? Not funded by a EU programme Is the Job related to staff position within a Research Infrastructure? No

Offer Description

Researcher for carrying out the prime editing experiments necessary for the publication of the article entitled “Highly Efficient Prime Editing Correction of a Prevalent Frameshift COL7A1 Mutation in Recessive Dystrophic Epidermolysis Bullosa

Graduate in Biology and Master's degree in Biotechnology

Specific Requirements

- Experience in developing and optimizing Base and Prime editing as gene editing strategies.




- Experience in the culture and maintenance of primary cells and cell lines
- Experience in the purification and isolation of skin cells from human and murine primary samples (skin biopsies).
- Experience in the molecular biology techniques, including plasmid amplification, cloning, genomic DNA, plasmid, protein, and RNA extraction, PCR, qPCR, ddPCR, Western blot, and in vitro messenger RNA synthesis.
- Experience in the characterization of generated mutations using next-generation sequencing and Sanger sequencing.
- Experience in the development of bioengineered skin equivalents.
- Experience in histological tissue processing, sectioning, and immunohistochemistry and immunofluorescence techniques.
- Experience in writing and orally presenting experimental results in English.

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📌 Predoctoral Researcher, Advanced Therapies (España)
🏢 Fundación Instituto Investigación Sanitaria Fundación Jimenez Diaz
📍 España