Job Description
We are looking for a Senior Research Technician to take ownership of a high-impact project on non-genetic drug resistance in melanoma—and we want someone who treats this as their own scientific mission. Your core task: develop and implement a physiologically relevant 3D model that captures the real-time crosstalk between tumor cells and the microenvironment, directly informing combination strategies to overcome treatment failure. We need a hands‑on expert in primary cell culture, multiplex assays, and 3D scaffolds—someone who troubleshoots independently, mentors effectively, and bridges lab work with translational goals.
Main Responsibilities

Establish and optimize a 3D co-culture model integrating melanoma cells with stromal/immune components to mimic the tumor microenvironment.
Perform drug sensitivity assays and longitudinal viability tests to monitor treatment response and acquired resistance.
Conduct molecular analyses (RNA/protein extraction, qPCR, Western blotting, cytokine profiling) to characterize non-genetic adaptive changes.




Carry out high-content imaging and quantitative microscopy to assess spatial interactions, invasion, and phenotypic plasticity within 3D constructs.
Maintain detailed electronic lab records, analyze data with statistical rigor, and present results in team meetings.
Train and supervise junior technicians/students, manage reagent inventory, and troubleshoot protocols proactively.
Collaborate with bioinformaticians to integrate wet‑lab data with omics datasets, feeding results into therapeutic strategy development.

Qualifications And Experience

Master in Biomedicine or closely related field, with a focus on cancer research.
Experience in tissue culture and data analysis
Strong experience in molecular and cellular biology techniques (DNA and RNA isolation, RNA quantification, qPCR and immunofluorescence).
Strong experience in cell culture, including the establishment of primary cultures.
Experience in proteomics, parti

📌 2026_112_SENIOR RESEARCH TECHNICIAN (Municipio de Badalona)
🏢 IMPPC, Institut de Medicina Predictiva i Personalitzada del Cancer
📍 Municipio de Badalona