We are looking for a Senior Research Technician to take ownership of a high-impact project on non-genetic drug resistance in melanoma—and we want someone who treats this as their own scientific mission. Your core task: develop and implement a physiologically relevant 3D model that captures the real-time crosstalk between tumor cells and the microenvironment, directly informing combination strategies to overcome treatment failure. We need a hands‑on expert in primary cell culture, multiplex assays, and 3D scaffolds someone who troubleshoots independently, mentors effectively, and bridges lab work with translational goals.
Main Responsibilities
- Establish and optimize a 3D co‑culture model integrating melanoma cells with stromal/immune components to mimic the tumor microenvironment.
- Perform drug sensitivity assays and longitudinal viability tests to monitor treatment response and acquired resistance.
- Conduct molecular analyses (RNA/protein extraction, qPCR, Western blotting, cytokine profiling) to characterize non‑genetic adaptive changes.




- Carry out high‑content imaging and quantitative microscopy to assess spatial interactions, invasion, and phenotypic plasticity within 3D constructs.
- Maintain detailed electronic lab records, analyze data with statistical rigor, and present results in team meetings.
- Train and supervise junior technicians/students, manage reagent inventory, and troubleshoot protocols proactively.
- Collaborate with bioinformaticians to integrate wet‑lab data with omics datasets, feeding results into therapeutic strategy development.

Qualifications And Experiencie
- Master in Biomedicine or closely related field, with a focus on cancer research.
- Experience in tissue culture and data analysis
- Strong experience in molecular and cellular biology techniques (DNA and RNA isolation, RNA quantification, qPCR and immunofluoresence).
- Strong experience in cell culture, including the establishment of primary cultures.
- Experience in proteomics,

📌 2026_112_SENIOR RESEARCH TECHNICIAN (España)
🏢 IMPPC, Institut de Medicina Predictiva i Personalitzada del Cancer
📍 España